In-vitro Anti-inflammatory and Antioxidant Activity of Onosma Hispidum (ratanjot) Roots

Aims: To evaluate the anti-inflammatory and antioxidant activity of methanolic root extract of onosma hispidum (Ratanjot) by in-vitro methods. Methods: DPPH free radical and ABTS radical scavenging activity estimation with total phenolic and flavonoid content methods were used to evaluate antioxidant activity while egg albumin denaturation method and HRBC membrane stabilization methods were used to estimate anti-inflammatory potential in-vitro. Results: Results concluded that methanolic root extract of O. hispidum possess highly significant anti-inflammatory and free radical scavenging property, having IC50 value 2.73 μg/ml by DPPH method while in ABTS radical scavenging activity the IC50 value was 2.74 μg/ml with the experimental value of standard ascorbic acid was 1.47 μg/ml. FolinCiocalteu method showed that extract exhibited significant total phenolic content 110.46 mg gallic acid equivalent/gm while total flavanoid content as rutin equivalents 114.83 mg per gm of extract. Conclusion: The plant exhibits significant anti-inflammatory and antioxidant activity as confirmed by IC50 values.